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个人信息

博士生导师
研究员
2003年获得“国家杰出青年基金”
Email: qin@cemps.ac.cn
个人网页:

研究方向

放线菌分子遗传学和基因组工程研究

覃重军

个人简介

1983年9月–1987年7月, 武汉大学, 生物系，微生物学专业, 理学学士
1987年9月–1992年12月, 华中农业大学, 微生物学, 理学博士, 导师: 陈华癸、周启、邓子新
1993年1月-1994年12月, 华中农业大学, 生命科技学院, 讲师
1994年12月-1995年7月, 华中农业大学, 生命科技学院, 副教授
1995年7月-2001年6月, 美国斯坦福大学遗传系, 博士后和访问学者, 导师: Stanley N. Cohen
2001年7月-至今, 中国科学院上海生命科学研究院, 植物生理生态研究所, 研究员，中科院合成生物学重点实验室主任。

研究工作

近年来，以原核生物模式菌株大肠杆菌和真核生物模式菌株酿酒酵母为材料，开展合成生物学前沿探索研究，包括构建简约化基因组、重要功能基因群的模块化、发展合成生物学使能技术等，尤其是尝试结合经典分子生物学的“假说驱动”和合成生物学的“工程化设计与实施”，回答一些重大的分子生物学基础科学问题。

主要成果

从事放线菌的分子遗传学研究与应用（1987年至今），系统地研究了放线菌线型质粒和环型质粒的生物学功能，包括复制、重组、修复、接合转移等。建立了粘细菌、高温链霉菌等遗传操作系统，构建了连续删除全部内源PKS/NRPS基因簇的天蓝色链霉菌底盘细胞。与浙江海正药业公司合作，利用基因组工程等技术构建与改良了多拉菌素和尼莫克丁工业菌株，打破了国外的垄断，2011-2014年产值约7.8亿元。

自2011年以来以大肠杆菌和酿酒酵母为材料，开展了合成生物学前沿探索研究。完成了大肠杆菌染色体全部复制基因等的人工成簇化、模块化和定量化测试，发展了MEGA、CasHRA等合成生物学使能技术，完成了国际首例人工创建一条线型和环型染色体的真核酿酒酵母。

在国内外发表论文80余篇，包括Nature, Nucleic Acids Research, Cell Research等国际重要刊物，申请或授权专利10多项。“基因组工程构建多拉菌素工业生产菌及其产业化技术”项目获2014年度上海市技术发明二等奖（排名第一）。目前承担国家基金委重点、上海市重大、中科院先导B等科研项目。

    1. Yangyang Shao, Ning Lu, Zhenfang Wu, Chen Cai, Shanshan Wang, Ling-Li Zhang, Fan Zhou, Shijun Xiao, Lin Liu, Xiaofei Zeng, Huajun Zheng, Chen Yang, Zhihu Zhao, Guoping Zhao*, Jinqiu Zhou*, Xiaoli Xue* and Zhongjun Qin* (2018) Creating a functional single chromosome yeast. Nature (article). 560:331–335.

    2. Yangyang Shao, Ning Lu, Chen Cai, Fan Zhou, Shanshan Wang, Zhihu Zhao*, Guoping Zhao*, Jinqiu Zhou*, Xiaoli Xue* and Zhongjun Qin* (2018). A single circular chromosome yeast. Cell Research. 10.1038/s41422-018-0110-y.

    3. Yangyang Shao, Ning Lu, Zhongjun Qin* and Xiaoli Xue* (2018). CRISPR-Cas9 facilitated multiple-chromosome fusion in Saccharomyces cerevisiae. ACS Synthetic Biology. 10.1021/acssynbio.8b00397.

    4. Yangyang Shao, Ning Lu, Xiaoli Xue* and Zhongjun Qin* (2018). CRISPR-Cas9 facilitated multiple-chromosome fusion in Saccharomyces cerevisiae. Nature Protocol. Invited.

    5. Jianting Zhou, Ronghai Wu, Xiaoli Xue* and Zhongjun Qin* (2016) CasHRA (Cas9-facilitated Homologous Recombination Assembly) method of constructing megabase-sized DNA. Nucleic Acids Research. 4(14):e124.

    6. Xiaoli Xue*, Tao Wang, Peng Jiang, Yangyang Shao, Min Zhou, Li Zhong, Ronghai Wu, Jianting Zhou, Haiyang Xia, Guoping Zhao and Zhongjun Qin* (2015) MEGA (Multiple Essential Genes Assembling) deletion and replacement method for genome reduction in Escherichia coli. ACS Synthetic Biology. 4(6):700–706.

    7. Jian Chen, Haiyang Xia*, Fujun Dang, Qingyu Xu, Wenjun Li and Zhongjun Qin* (2015) Characterization of the chromosomal integration of Saccharopolyspora plasmid pCM32 and its application to improve production of spinosyn in Saccharopolyspora spinosa. Applied Microbiology and Biotechnology. 99:10141–10149.

    8. Yalan Chen, Huarong Tan and Zhongjun Qin* (2013) Characterization of a replication locus and formation of a higher-order complex between RepA protein and two inverted-repeats in Streptomyces plasmid pSV1. FEMS Microbiology Letters. 349: 144–152.

    9. Shiyuan Peng, Ana Zeng, Li Zhong, Ran Zhang, Min Zhou, Qiuxiang Cheng, Liqian Zhao, Tao Wang, Huarong Tan and Zhongjun Qin* (2013) Three functional replication origins of the linear and artificially-circularized plasmid SCP1 of Streptomyces coelicolor. Microbiology. 159: 2127–2140.

    10. Ran Zhang, Haiyang Xia, Qingyu Xu, Fujun Dang and Zhongjun Qin* (2013) Recombinational cloning of the antibiotic biosynthetic gene clusters in linear plasmid SCP1 of Streptomyces coelicolor A3(2). FEMS Microbiology Letters. 345: 39–48.

    11. Pengfei Xie, Ana Zeng and Zhongjun Qin* (2013) A putative transglycosylase encoded by SCO4132 influences morphological differentiation and actinorhodin production in Streptomyces coelicolor. Acta Biochimica et Biophysica Sinica. 45(4):296–302.

    12. Tao Wang, Zhenhua Chen, Qiuxiang Cheng, Min Zhou, Xinli Tian, Pengfei Xie, Li Zhong, Meijuan Shen and Zhongjun Qin* (2012) Characterization of replication and conjugation of plasmid pWTY27 from a widely distributed Streptomyces species. BMC Microbiology. 12:253.

    13. Zhenhua Chen, Li Zhong, Meijuan Shen, Ping Fang and Zhongjun Qin* (2012) Characterization of Streptomyces plasmid-phage pFP4 and its evolutionary implications. Plasmid. 68:170–178.

    14. Min Zhou, Xinyun Jing, Pengfei Xie, Weihua Chen, Tao Wang, Haiyang Xia and Zhongjun Qin* (2012) Sequential deletion of all the PKS and NRPS biosynthetic gene clusters and a 900-kb subtelomeric sequence of the linear chromosome of Streptomyces coelicolor. FEMS Microbiology Letters. 333(2):169–179.

    15. Yun Fan, Yumei Dai, Qiuxiang Cheng, Guangjun Zhang, Dongshu Zhang, Ping Fang*, Hang Wu, Linquan Bai*, Zixin Deng and Zhongjun Qin* (2012) A self-ligation method for PCR-sequencing the telomeres of Streptomyces and Mycobacterium linear replicons. Journal of Microbiological Methods. 90:105–107.

    16. Weihua Chen and Zhongjun Qin* (2011) Development of a gene cloning system in a fast-growing and moderately thermophilic Streptomyces species and heterologous expression of Streptomyces antibiotic biosynthetic gene clusters. BMC Microbiology. 11(1):243.

    17. Ana Zeng, Tao Wang, Haiyang Xia, Shiyuan Peng, Weihua Chen, Chenglin Jiang, Lihua Xu, Li Zhong, Meijuan Shen and Zhongjun Qin* (2011) Development of a vector and host system and characterization of replication of plasmid pSQ10 in moderately halophilic Nocardiopsis. Acta Biochimica et Biophysica Sinica. 43(9):738–743.

    18. Peng Guo, Qiuxiang Cheng, Pengfei Xie, Yun Fan, Weihong Jiang and Zhongjun Qin* (2011) Characterization of the multiple CRISPR loci on Streptomyces linear plasmid pSHK1. Acta Biochimica et Biophysica Sinica. 43(8):630–639.

    19. Li Zhong, Qiuxiang Cheng, Xinli Tian, Liqian Zhao and Zhongjun Qin* (2010) Characterization of the replication, transfer and plasmid/lytic phage cycle of the Streptomyces plasmid-phage pZL12. Journal of Bacteriology. 192:3747–3754.

    20. Ran Zhang, Shiyuan Peng and Zhongjun Qin* (2010) Two internal origins of replication on Streptomyces linear plasmid pFRL1. Applied and Environmental Microbiology. 76(17):5676–5683.

    21. Zhiqun Lu, Pengfei Xie and Zhongjun Qin* (2010) Promotion of markerless deletion of the actinorhodin biosynthetic gene cluster in Streptomyces coelicolor. Acta Biochimica et Biophysica Sinica. 42(10):717–721.

    22. Pengfei Xie, Ana Zeng and Zhongjun Qin* (2009) cmdABCDEF, a cluster of genes encoding membrane proteins for differentiation and antibiotic production in Streptoymyces coelicolor A3(2). BMC Microbiology. 9:157.

    23. Yongqiang Tian, Weihong Jiang, Guoping Zhao* and Zhongjun Qin* (2009) In vivo conjugation-coupled recombinational cloning of a Streptomyces lividans chromosomal telomeric DNA using a linear plasmid. Biotechnology Letter. 31(8):1253–1258.

    24. Ran Zhang, Haiyang Xia, Peng Guo and Zhongjun Qin* (2009) Variation in the replication loci of Streptomyces linear plasmids. FEMS Microbiology Letters. 290:209–216.

    25. Ran Zhang, Ana Zeng, Ping Fang and Zhongjun Qin* (2008) Characterization of the replication and conjugation loci of Streptomyces circular plasmids pFP11 and pFP1 and their ability to propagate in linear mode with artificially attached telomeres. Applied and Environmental Microbiology. 74(11):3368–3376.

    26. Jingyi Zhao, Li Zhong, Meijuan Shen, Zhijie Xia, Qiuxiang Cheng, Xia Sun, Guoping Zhao, Yuezhong Li* and Zhongjun Qin* (2008) Discovery of an autonomously-replicating plasmid pMF1 from Myxococcus fulvus and the development of a gene cloning system in Myxococcus Xanthus. Applied and Environmental Microbiology. 74(7):1980–1987.

    27. Mingxuan Xu, Yingmin Zhu, Ran Zhang, Meijuan Shen, Weihong Jiang, Guoping Zhao* and Zhongjun Qin* (2006) Characterization of the genetic components of Streptomyces lividans linear plasmid SLP2 for replication in circular and linear modes. Journal of Bacteriology.188:6851–6857.

    28. Ran Zhang, YongYang, Ping Fang, Chenglin Jiang, Lihua Xu, Yingmin Zhu, Meijuan Shen, Haiyang Xia, Jianfu Zhao, Tongbing Chen and Zhongjun Qin* (2006) Diversity of telomere palindromic sequences and replication genes among Streptomyces linear plasmids. Applied and Environmental Microbiology. 72(9):5728–5733.

    29. Meijuan Shen, Ping Fang, Deqiang Xu, Yalei Zhang, Weihuan Cao, Yingmin Zhu, JianfuZhao and Zhongjun Qin* (2006) Replication and Inheritance of Nocardia Plasmid pC1. FEMS Microbiology Letters. 261:47–52.

    30. Haiyang Xia, Yongqiang Tian, Ran Zhang, Kaichun Lin and Zhongjun Qin* (2006) Characterization of Nocardia Plasmid pXT107. Acta Biochimica et Biophysica Sinica. 38(9):620–624.

    31. Mingxuan Xu, Yingmin Zhu, Meijuan Shen, Weihong Jiang, Guoping Zhao* and Zhongjun Qin* (2006) Characterization of the essential genetic components for conjugal transfer of Streptomyces lividans linear plasmid SLP2. Progress in Biochemistry and Biophysics. 33(10):1–8.

    32. Yongqiang Tian, Pei Hao, G.uoping Zhao* and Zhongjun Qin* (2005) Cloning and characterization of the chromosomal replication origin region of Amycolatopsis mediterranei U32. Biochemical and Biophysical Research Communications. 333(1):14-20.

    33. Zhongjun Qin, Meijuan Shen and Stanley N. Cohen* (2003) Identification and characterization of a pSLA2 plasmids locus required for linear DNA replication and circular plasmids stable inheritance in Streptomyces lividans. Journal of Bacteriology. 185 (22):6575–6582.

    34. Zhongjun Qin and Stanley N. Cohen* (2002) Survival mechanisms for Streptomyces linear replicons after telomere damage. Molecular Microbiology. 45(3):785–794.

    35. Zhongjun Qin and Stanley N. Cohen* (2000) Long palindrome formed in Streptomyces by nonrecombinational intra-strand annealing. Genes&Development. 14:1789–1796.

    36. Zhongjun Qin and Stanley N. Cohen* (1998) Replication at the telomeres of Streptomyces linear plasmid pSLA2. Molecular Microbiology. 28:893–903.

    37. Zhongjun Qin, Kaiman Peng, Xiufen Zhou, Rongfang Liang, Qi Zhou, Huakui Chen, David A. Hopwood, Tobias Kieser and Zixin Deng* (1994) Development of a gene cloning system for Streptomyces hygroscopicus subsp. yingchenggenesis, a producer of the three useful antifungal compounds, by elimination of three barriers to DNA transfer. Journal of Bacteriology. 176:2090–2095.