首页　　 人才队伍
个人信息

博士生导师
研究员

Email: huangcf@cemps.ac.cn
个人网页: https://www.researchgate.net/profile/Chao-Feng-Huang

研究方向

植物金属逆境生物学

黄朝锋

个人简介

2016年12月-至今，中科院分子植物科学卓越创新中心，研究员
2012年8月-2016年11月，南京农业大学资源与环境科学学院，教授
2012年1月-2012年7月，中科院上海植物生理生态研究所，副研究员
2011年1月-2011年12月，美国普渡大学，博士后
2010年9月-2010年12月，美国加州大学河滨分校，博士后
2009年10月-2010年8月，日本冈山大学资源植物科学研究所，特别契约助理研究员
2006年10月-2009年9月，日本爱媛大学，博士
2004年10月-2006年9月，日本香川大学，硕士
2003年7月-2004年6月，中科院上海植物生理生态研究，研究助理
2000年9月-2003年6月，华南农业大学，硕士
1996年9月-2000年6月，华南农业大学，学士

研究工作

本研究组主要从事植物抗铝毒机制和锰利用机制的研究工作

1、植物抗铝毒机制的研究
铝在地壳中是最丰富的金属元素，约占8%。在中性或碱性土壤中，铝主要以不可溶的硅酸铝或氧化铝形式存在，对植物不会产生毒害。但是在酸性土壤中，部分三价铝离子（Al3+）被溶解出抑制植物根的生长，进而影响作物的生长发育，最终导致作物减产。因此，铝毒是作物在酸性土壤生产的主要限制因子，也是仅次于干旱的第二大非生物逆境，并且随着过度频繁地耕作和氮肥的过度使用土壤有进一步酸化的趋势，这使得铝毒问题变得日益严重。为了应对酸性土壤的铝毒害，植物进化了一系列的抗铝毒机制，其中以锌指转录因子STOP1/ART1为核心的表达调控是植物中保守的和关键的抗铝毒机制。本课题主要通过正向遗传筛选、蛋白互作筛选、磷酸化组学等手段解析STOP1/ART1的转录后调控机制及其上游铝毒感受和信号转导机制，并鉴定抗铝毒新基因，揭示植物抗铝毒新机制，为改良作物抗铝毒提供新思路和重要基因资源。

2、植物锰、镉运输与调控机制的研究
锰参与植物光合、酶氧化还原、脂类合成等过程，是植物必需的矿质营养元素，而重金属镉是对植物和人类都具有较大毒害的矿质元素。由于镉不是植物的必需元素，植物没有进化出专门运输镉的转运蛋白。研究表明，镉主要通过锰吸收途径进入植物体内。本课题组主要研究植物对锰的运输与调控机制，并利用对锰运输与调控机制的理解阻控作物对镉的积累，保障作物的食品安全。

主要成果

    Publications (# Co-first author, * Corresponding author)


    2024

    39. Wei X#, Zhu YF#, Xie WX, Ren WW, Zhang Y, Zhang H, Dai SJ, and Huang CF* (2024) H2O2 negatively regulates aluminum resistance via oxidation and degradation of the STOP1 transcription factor. Plant Cell 36: 688-708.

    2023

    38. Luo ZL, Liu CL, Yang XF, Zhu JK, and Huang CF* (2023) Mitigating cadmium accumulation in rice without compromising growth via modifying the regulatory region of OsNRAMP5. Stress Biology 3: 34.

    37. Xie WX, Liu S, Gao HL, Wu J, Liu DL, Kinoshita T, and Huang CF* (2023) A PP2C.D phosphatase SAL1 positively regulates aluminum resistance via restriction of aluminum uptake in rice. Plant Physiology (Accepted)

    36. Zhou FL, Somesh S, Zhang J, Fang Q, Li CY, Wang JW, Zhao CZ, Wang PC, and Huang CF* (2023) The MEKK1-MKK1/2-MPK4 cascade phosphorylates and stabilizes STOP1 to confer aluminum resistance in Arabidopsis. Molecular Plant 16: 1-17.

    2022

    35. Huang CF* (2022) Ca2+ signaling in plant manganese uptake: CPK21/23 kinases phosphorylate and activate manganese transporter NRAMP1. Stress Biology 2: 43. 

    34. Li L#, Zhu ZZ#, Liao YH #, Yang CH, Fan N, Zhang J, Yamaji N, Dirick L, Jian Feng Ma, Curie C, and Huang CF* (2022) NRAMP6 and NRAMP1 cooperatively regulate root growth and manganese translocation under manganese deficiency in Arabidopsis. Plant Journal 110: 1564-1577.

    33. Zhu X#, Wang P#, Bai ZM, Herde M, Ma YQ, Li N, Liu S, Huang CF, Cui RX, Ma HY, Zhang M, Wang H, Wei TD, Quan TY, Zhang W, Liu CG, Zhang T, and Yang ZB* (2022) Calmodulin-like protein CML24 interacts with CAMTA2 and WRKY46 to regulate ALMT1-dependent Al resistance in Arabidopsis thaliana. New Phytologist 233: 2471-2487.

    2021

    32. Yang CH#, Wang C#, Singh S#, Fan N, Liu S, Zhao L, Cao HL, Xie WX, Yang CW* and Huang CF* (2021) Golgi‐localised manganese transporter PML3 regulates Arabidopsis growth through modulating Golgi glycosylation and cell wall biosynthesis. New Phytologist 231: 2200-2214.

    31. Zhu YF#, Guo J#, Zhang Y, and Huang CF* (2021) The THO/TREX complex component RAE2/TEX1 is involved in the regulation of aluminum resistance and low phosphate response in Arabidopsis. Frontiers in Plant Science 12:698443.

    30. Huang CF* (2021) Activation and activity of STOP1 in aluminum resistance. Journal of Experimental Botany 72: 2269-2272.

    29. Fang Q, Zhang J, Yang DL, and Huang CF* (2021) The SUMO E3 ligase SIZ1 partially regulates STOP1 SUMOylation and stability in Arabidopsis thaliana. Plant Signaling & Behavior 16 (5): 1899487.

    28. Fang Q#, Zhou FL#, Zhang Y, Singh S, and Huang CF* (2021) Degradation of STOP1 mediated by the F‐box proteins RAH1 and RAE1 balances aluminum resistance and plant growth in Arabidopsis thaliana. Plant Journal 106:493-506.

    2020

    27. Fang Q#, Zhang J#, Zhang Y, Fan N, van den Burg HA, and Huang CF* (2020) Regulation of aluminum-resistance in Arabidopsis involves the SUMOylation of the zinc finger transcription factor STOP1. Plant Cell 32: 3921-3938.

    26. Liu S, Zhao L, Liao YH, Luo ZL, Wang H, Wang P, Zhao H, Xia JX, and Huang CF* (2020) Dysfunction of the 4-Coumarate:Coenzyme A Ligase 4CL4 impacts aluminum resistance and lignin accumulation in rice. Plant Journal 104:1233-1250.

    25. Guo JL, Zhang Y, Gao HL, Li SB, Wang ZY, and Huang CF* (2020) Mutation of HPR1 encoding a component of the THO/TREX complex reduces STOP1 accumulation and aluminum resistance in Arabidopsis thaliana. New Phytologist 228: 179-193

    24. Liu CL#, Gao ZY#, Shang LG#, Yang CH, Ruan BP, Zeng DL, Guo LB, Zhao FJ, Huang CF*, and Qian Q* (2020) Natural variation in the promoter of OsHMA3 contributes to differential grain cadmium accumulation between Indica and Japonica rice. Journal of Integrative Plant Biology 62: 314-329

    2019

    23. Zhang Y#, Zhang J#, Guo JL#, Zhou FL, Singh S, Xu X, Xie Q, Yang ZB, and Huang CF* (2019) F-box protein RAE1 regulates the stability of the aluminum-resistance transcription factor STOP1 in Arabidopsis. PNAS 116: 319-327

    22. Yang CH#, Zhang Y# and Huang CF*(2019) Reduction in cadmium accumulation in japonica rice grains by CRISPR/Cas9-mediated editing of OsNRAMP5. Journal of Integrative Agriculture 18:688-697.

    2018

    21. Zhu HF#, Xie WX#, Xu DC#, Miki D, Tang K, Huang CF*, and Zhu JK* (2018) DNA demethylase ROS1 negatively regulates the imprinting of DOGL4 and seed dormancy in Arabidopsis thaliana. PNAS 115: E9962-E9970.

    20. Zhang Y#, Guo JL#, Chen M, Li L, Wang LH, and Huang CF* (2018) The cell cycle checkpoint regulator ATR is required for internal aluminum toxicity-mediated root growth inhibition in Arabidopsis. Frontiers in Plant Science 9:118.

    19. Gao HL, Xie WX, Yang CH, Xu JY, Li JJ, Wang H, Chen X, and Huang CF* (2018) NRAMP2, a trans-Golgi network localized manganese transporter, is required for Arabidopsis root growth under manganese deficiency. New Phytologist 217:179-193.

    2014-2016

    18. Liu S, Gao HL, Wu XY, Fang Q, Chen L, Zhao FJ, and Huang CF* (2016) Isolation and characterization of an aluminum-resistant mutant in rice. Rice 9:60.

    17. Yan JL, Wang PT, Wang P, Yang M, Lian XM, Tang Z, Huang CF, Salt DE, and Zhao FJ (2016) A loss-of-function allele of OsHMA3 associated with high cadmium accumulation in shoots and grain of Japonica rice cultivars. Plant Cell & Environment 39:1941–1954.

    16. Yang Y, Liu Y, Huang CF, Silva J, and Zhao FJ (2016) Aluminium alleviates fluoride toxicity in tea (Camellia sinensis). Plant Soil 402:179-190.

    15. Zhu HF, Wang H, Zhu YF, Zou JW, Zhao FJ, and Huang CF* (2015) Genome-wide transcriptomic and phylogenetic analyses reveal distinct aluminum-tolerance mechanisms in the aluminum-accumulating species buckwheat (Fagopyrum tataricum). BMC Plant Biology 15:16.

    14. Yang M, Zhang YY, Zhang LJ, Hu JT, Zhang X, Lu K, Dong HX, Wang DJ, Zhao FJ, Huang CF, and Lian XM (2014) OsNRAMP5 contributes to manganese translocation and distribution in rice shoots. Journal of Experimental Botany 65: 4849–4861.

    13. Huang CF* and Zhu JK* (2014) RNA splicing factors and RNA-directed DNA methylation. Biology 3:243-254.

    Before 2014

    12. Huang CF, Miki D, Tang K, Zhou HR, Zheng Z, Chen W, Ma ZY, Yang L, Zhang H, Liu R, He XJ, Zhu JK (2013) A pre-mRNA-splicing factor is required for RNA-directed DNA methylation in Arabidopsis. PLOS Genetics 9: e1003779.

    11. Dou K#, Huang CF#, Ma ZY, Zhang CJ, Zhou JX, Huang HW, Cai T, Tang K, Zhu JK, and He XJ (2013) The PRP6-like splicing factor STA1 is involved in RNA-directed DNA methylation by facilitating the production of Pol V-dependent scaffold RNAs. Nucleic Acids Research 41: 8489-8502.

    10. Tsutsui T, Yamaji N, Huang CF, Motoyama R, Nagamura Y, Ma JF (2012) Comparative genome-wide transcriptional analysis of Al-responsive genes reveals novel Al tolerance mechanisms in rice. PLOS ONE 7: e48197.

    9. Huang CF, Yamaji N, Chen ZC, and Ma JF (2012) A tonoplast-localized half-size ABC transporter is required for internal detoxification of Al in rice. Plant Journal 69: 857-867.

    8. Huang CF, Yamaji N, Ono K, and Ma JF (2012) A leucine-rich repeat receptor-like kinase gene is involved in the specification of outer cell layers in rice roots. Plant Journal 69: 565–576 (Cover story).

    7. Ueno D, Yamaji N, Kono I, Huang CF, Ando T, Yano M, and Ma JF (2010) Gene limiting cadmium accumulation in rice. PNAS 107: 16500-16505.

    6. Huang CF, Yamaji N, and Ma JF (2010) Knockout of a bacterial-type ATP-binding cassette transporter gene, AtSTAR1, results in increased aluminum sensitivity in Arabidopsis. Plant Physiology 153: 1669-1677.

    5. Yamaji N#, Huang CF#, Nagao S, Yano M, Sato Y, Nagamura Y, and Ma JF (2009) A zinc finger transcription factor ART1 regulates multiple genes implicated in aluminum tolerance in rice. Plant Cell 21: 3339-3349.

    4. Huang CF, Yamaji N, Nishimura M, Tajima S, and Ma JF (2009) A rice mutant sensitive to Al toxicity is defective in the specification of root outer cell layers. Plant and Cell Physiology 50: 976 -985.

    3. Wang LP, Yin HF, Qian Q, Yang J, Huang CF, Hu XH, and Luo D (2009) NECK LEAF 1, a GATA type transcription factor, modulates organogenesis by regulating the expression of multiple regulatory genes during reproductive development in rice. Cell Research 19: 598-611.

    2. Huang CF#, Yamaji N#, Mitani N, Yano M, Nagamura Y, and Ma JF (2009) A bacterial-type ABC transporter is involved in aluminum tolerance in rice. Plant Cell 21: 655-667.

    1. Ma JF, Nagao S, Huang CF, and Nishimura M (2005) Isolation and characterization of a rice mutant hypersensitive to Al. Plant and Cell Physiology 46: 1054-1061.